Time-resolved neurite mechanics by thermal fluctuation assessments

Fernanda Gárate, Timo Betz, Maria Pertusa, Roberto Bernal

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

In the absence of simple noninvasive measurements, the knowledge of temporal and spatial variations of axons mechanics remains scarce. By extending thermal fluctuation spectroscopy (TFS) to long protrusions, we determine the transverse amplitude thermal fluctuation spectra that allow direct and simultaneous access to three key mechanics parameters: axial tension, bending flexural rigidity and plasma membrane tension. To test our model, we use PC12 cell protrusions - a well-know biophysical model of axons - in order to simplify the biological system under scope. For instance, axial and plasma membrane tension are found in the range of nano Newton and tens of pico Newtons per micron respectively. Furthermore, our results shows that the TFS technique is capable to distinguish quasi-identical protrusions. Another advantage of our approach is the time resolved nature of the measurements. Indeed, in the case of long term experiments on PC12 protrusions, TFS has revealed large temporal, correlated variations of the protrusion mechanics, displaying extraordinary feedback control over the axial tension in order to maintain a constant tension value.

LanguageEnglish
Article number066020
JournalPhysical Biology
Volume12
Issue number6
DOIs
StatePublished - 30 Dec 2015

Fingerprint

Neurites
Mechanics
Hot Temperature
Spectrum Analysis
Axons
Cell Membrane
PC12 Cells

Keywords

  • neurite mechanics
  • noise spectroscopy
  • novel instrumentation

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Structural Biology

Cite this

Time-resolved neurite mechanics by thermal fluctuation assessments. / Gárate, Fernanda; Betz, Timo; Pertusa, Maria; Bernal, Roberto.

In: Physical Biology, Vol. 12, No. 6, 066020, 30.12.2015.

Research output: Contribution to journalArticle

@article{ccffc3109b9f4ed6807642159015875d,
title = "Time-resolved neurite mechanics by thermal fluctuation assessments",
abstract = "In the absence of simple noninvasive measurements, the knowledge of temporal and spatial variations of axons mechanics remains scarce. By extending thermal fluctuation spectroscopy (TFS) to long protrusions, we determine the transverse amplitude thermal fluctuation spectra that allow direct and simultaneous access to three key mechanics parameters: axial tension, bending flexural rigidity and plasma membrane tension. To test our model, we use PC12 cell protrusions - a well-know biophysical model of axons - in order to simplify the biological system under scope. For instance, axial and plasma membrane tension are found in the range of nano Newton and tens of pico Newtons per micron respectively. Furthermore, our results shows that the TFS technique is capable to distinguish quasi-identical protrusions. Another advantage of our approach is the time resolved nature of the measurements. Indeed, in the case of long term experiments on PC12 protrusions, TFS has revealed large temporal, correlated variations of the protrusion mechanics, displaying extraordinary feedback control over the axial tension in order to maintain a constant tension value.",
keywords = "neurite mechanics, noise spectroscopy, novel instrumentation",
author = "Fernanda G{\'a}rate and Timo Betz and Maria Pertusa and Roberto Bernal",
year = "2015",
month = "12",
day = "30",
doi = "10.1088/1478-3975/12/6/066020",
language = "English",
volume = "12",
journal = "Physical Biology",
issn = "1478-3967",
publisher = "Institute of Physics Publishing",
number = "6",

}

TY - JOUR

T1 - Time-resolved neurite mechanics by thermal fluctuation assessments

AU - Gárate,Fernanda

AU - Betz,Timo

AU - Pertusa,Maria

AU - Bernal,Roberto

PY - 2015/12/30

Y1 - 2015/12/30

N2 - In the absence of simple noninvasive measurements, the knowledge of temporal and spatial variations of axons mechanics remains scarce. By extending thermal fluctuation spectroscopy (TFS) to long protrusions, we determine the transverse amplitude thermal fluctuation spectra that allow direct and simultaneous access to three key mechanics parameters: axial tension, bending flexural rigidity and plasma membrane tension. To test our model, we use PC12 cell protrusions - a well-know biophysical model of axons - in order to simplify the biological system under scope. For instance, axial and plasma membrane tension are found in the range of nano Newton and tens of pico Newtons per micron respectively. Furthermore, our results shows that the TFS technique is capable to distinguish quasi-identical protrusions. Another advantage of our approach is the time resolved nature of the measurements. Indeed, in the case of long term experiments on PC12 protrusions, TFS has revealed large temporal, correlated variations of the protrusion mechanics, displaying extraordinary feedback control over the axial tension in order to maintain a constant tension value.

AB - In the absence of simple noninvasive measurements, the knowledge of temporal and spatial variations of axons mechanics remains scarce. By extending thermal fluctuation spectroscopy (TFS) to long protrusions, we determine the transverse amplitude thermal fluctuation spectra that allow direct and simultaneous access to three key mechanics parameters: axial tension, bending flexural rigidity and plasma membrane tension. To test our model, we use PC12 cell protrusions - a well-know biophysical model of axons - in order to simplify the biological system under scope. For instance, axial and plasma membrane tension are found in the range of nano Newton and tens of pico Newtons per micron respectively. Furthermore, our results shows that the TFS technique is capable to distinguish quasi-identical protrusions. Another advantage of our approach is the time resolved nature of the measurements. Indeed, in the case of long term experiments on PC12 protrusions, TFS has revealed large temporal, correlated variations of the protrusion mechanics, displaying extraordinary feedback control over the axial tension in order to maintain a constant tension value.

KW - neurite mechanics

KW - noise spectroscopy

KW - novel instrumentation

UR - http://www.scopus.com/inward/record.url?scp=84953775123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953775123&partnerID=8YFLogxK

U2 - 10.1088/1478-3975/12/6/066020

DO - 10.1088/1478-3975/12/6/066020

M3 - Article

VL - 12

JO - Physical Biology

T2 - Physical Biology

JF - Physical Biology

SN - 1478-3967

IS - 6

M1 - 066020

ER -