Effect of yeast (Xanthophyllomyces dendrorhous) and plant (Saint John's wort, lemon balm, and rosemary) extract based functional diets on antioxidant and immune status of Atlantic salmon (Salmo salar) subjected to crowding stress

S. Reyes-Cerpa, E. Vallejos-Vidal, M.J. Gonzalez-Bown, J. Morales-Reyes, D. Pérez-Stuardo, D. Vargas, M. Imarai, V. Cifuentes, E. Spencer, F.E. Reyes-López, Ana M. Sandino Garcia, Ana M. Sandino Garcia

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Abstract

Salmon farming may face stress due to the intensive culture conditions with negative impacts on overall performance. In this aspect, functional feed improves not only the basic nutritional requirements but also the health status and fish growth. However, to date no studies have been carried out to evaluate the effect of functional diets in salmon subjected to crowding stress. Thus, the aim of this study was to evaluate the effect of yeast extract (Xanthophyllomyces dendrorhous; diet A) and the combination of plant extracts (common Saint John's wort, lemon balm, and rosemary; diet B) on the antioxidant and immune status of Atlantic salmon grown under normal cultured conditions and then subjected to crowding stress. Fish were fed with functional diets during 30 days (12 kg/m3) and then subjected to crowding stress (20 kg/m3) for 10 days. The lipid peroxidation in gut showed that both diets induced a marked decrease on oxidative damage when fish were subjected to crowding stress. The protein carbonylation in muscle displayed at day 30 a marked decrease in both functional diets that was more marked on the stress condition. The expression of immune markers (IFNγ, CD4, IL-10, TGF-β, IgMmb, IgMsec, T-Bet, and GATA-3) indicated the upregulation of those associated to humoral-like response (CD4, IL-10, GATA-3) when fish were subjected to crowding stress. These results were confirmed with the expression of secreted IgM. Altogether, these functional diets improved the antioxidant status and increased the expression of genes related to Th2-like response suggesting a protective role on fish subjected to crowding stress. © 2017
LanguageEnglish
Pages250-259
Number of pages10
JournalFish and Shellfish Immunology
Volume74
DOIs
Publication statusPublished - 2018

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lemon balm
rosemary
Nutrition
Yeast
antioxidant
Salmo salar
yeast
Antioxidants
diet
yeasts
antioxidants
Fish
extracts
fish
interleukin-10
salmon
Interleukin-10
Carbonylation
intensive culture
nutritional requirement

Keywords

  • Atlantic salmon
  • Crowding stress
  • Functional feed
  • Immune response
  • Oxidative stress
  • Plant extract
  • Yeast

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Effect of yeast (Xanthophyllomyces dendrorhous) and plant (Saint John's wort, lemon balm, and rosemary) extract based functional diets on antioxidant and immune status of Atlantic salmon (Salmo salar) subjected to crowding stress. / Reyes-Cerpa, S.; Vallejos-Vidal, E.; Gonzalez-Bown, M.J.; Morales-Reyes, J.; Pérez-Stuardo, D.; Vargas, D.; Imarai, M.; Cifuentes, V.; Spencer, E.; Reyes-López, F.E.; Sandino Garcia, Ana M.; Sandino Garcia, Ana M.

In: Fish and Shellfish Immunology, Vol. 74, 2018, p. 250-259.

Research output: Contribution to journalArticle

Reyes-Cerpa, S. ; Vallejos-Vidal, E. ; Gonzalez-Bown, M.J. ; Morales-Reyes, J. ; Pérez-Stuardo, D. ; Vargas, D. ; Imarai, M. ; Cifuentes, V. ; Spencer, E. ; Reyes-López, F.E. ; Sandino Garcia, Ana M. ; Sandino Garcia, Ana M. / Effect of yeast (Xanthophyllomyces dendrorhous) and plant (Saint John's wort, lemon balm, and rosemary) extract based functional diets on antioxidant and immune status of Atlantic salmon (Salmo salar) subjected to crowding stress. In: Fish and Shellfish Immunology. 2018 ; Vol. 74. pp. 250-259.
@article{49f372211212472fa55d8b7b2110cad9,
title = "Effect of yeast (Xanthophyllomyces dendrorhous) and plant (Saint John's wort, lemon balm, and rosemary) extract based functional diets on antioxidant and immune status of Atlantic salmon (Salmo salar) subjected to crowding stress",
abstract = "Salmon farming may face stress due to the intensive culture conditions with negative impacts on overall performance. In this aspect, functional feed improves not only the basic nutritional requirements but also the health status and fish growth. However, to date no studies have been carried out to evaluate the effect of functional diets in salmon subjected to crowding stress. Thus, the aim of this study was to evaluate the effect of yeast extract (Xanthophyllomyces dendrorhous; diet A) and the combination of plant extracts (common Saint John's wort, lemon balm, and rosemary; diet B) on the antioxidant and immune status of Atlantic salmon grown under normal cultured conditions and then subjected to crowding stress. Fish were fed with functional diets during 30 days (12 kg/m3) and then subjected to crowding stress (20 kg/m3) for 10 days. The lipid peroxidation in gut showed that both diets induced a marked decrease on oxidative damage when fish were subjected to crowding stress. The protein carbonylation in muscle displayed at day 30 a marked decrease in both functional diets that was more marked on the stress condition. The expression of immune markers (IFNγ, CD4, IL-10, TGF-β, IgMmb, IgMsec, T-Bet, and GATA-3) indicated the upregulation of those associated to humoral-like response (CD4, IL-10, GATA-3) when fish were subjected to crowding stress. These results were confirmed with the expression of secreted IgM. Altogether, these functional diets improved the antioxidant status and increased the expression of genes related to Th2-like response suggesting a protective role on fish subjected to crowding stress. {\circledC} 2017",
keywords = "Atlantic salmon, Crowding stress, Functional feed, Immune response, Oxidative stress, Plant extract, Yeast",
author = "S. Reyes-Cerpa and E. Vallejos-Vidal and M.J. Gonzalez-Bown and J. Morales-Reyes and D. P{\'e}rez-Stuardo and D. Vargas and M. Imarai and V. Cifuentes and E. Spencer and F.E. Reyes-L{\'o}pez and {Sandino Garcia}, {Ana M.} and {Sandino Garcia}, {Ana M.}",
note = "Export Date: 13 June 2018 CODEN: FSIME Correspondence Address: Sandino, A.M.; Laboratorio de Virolog{\'i}a, Centro de Biotecnolog{\'i}a Acu{\'i}cola, Departamento de Biolog{\'i}a, Facultad de Qu{\'i}mica y Biolog{\'i}a, Universidad de Santiago de Chile, Alameda Libertador Bernardo O'Higgins 3363, Estaci{\'o}n Central, Correo 40, casilla 33, Chile; email: ana.sandino@usach.cl Funding details: 74170091 Funding details: 11150807, FONDECYT, Fondo Nacional de Desarrollo Cient{\'i}fico, Tecnol{\'o}gico y de Innovaci{\'o}n Tecnol{\'o}gica Funding details: 1130882, FONDECYT, Fondo Nacional de Desarrollo Cient{\'i}fico, Tecnol{\'o}gico y de Innovaci{\'o}n Tecnol{\'o}gica Funding details: AMS, Agricultural Marketing Service Funding details: OI101205 Funding details: SRC, Semiconductor Research Corporation Funding details: 09MCSS-6691 Funding details: 09MCSS-6698 Funding details: ES Funding text: This study was supported by INNOVA-CORFO (No. 09MCSS-6691 and 09MCSS-6698 ), FONDECYT (No. 1130882 ; 11150807 ), DICYT-USACH , and VRIDEI-USACH (MI, ES, AMS); Universidad Mayor start-Up OI101205 funds to SRC, and the grants from CONICYT-BCH (Chile) Postdoctoral fellowship (No. 74170091 ) to EVV, and VRIDEI-USACH (living/travel allowance) to FERL. 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year = "2018",
doi = "10.1016/j.fsi.2017.12.061",
language = "English",
volume = "74",
pages = "250--259",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Caister Academic Press",

}

TY - JOUR

T1 - Effect of yeast (Xanthophyllomyces dendrorhous) and plant (Saint John's wort, lemon balm, and rosemary) extract based functional diets on antioxidant and immune status of Atlantic salmon (Salmo salar) subjected to crowding stress

AU - Reyes-Cerpa, S.

AU - Vallejos-Vidal, E.

AU - Gonzalez-Bown, M.J.

AU - Morales-Reyes, J.

AU - Pérez-Stuardo, D.

AU - Vargas, D.

AU - Imarai, M.

AU - Cifuentes, V.

AU - Spencer, E.

AU - Reyes-López, F.E.

AU - Sandino Garcia, Ana M.

AU - Sandino Garcia, Ana M.

N1 - Export Date: 13 June 2018 CODEN: FSIME Correspondence Address: Sandino, A.M.; Laboratorio de Virología, Centro de Biotecnología Acuícola, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Alameda Libertador Bernardo O'Higgins 3363, Estación Central, Correo 40, casilla 33, Chile; email: ana.sandino@usach.cl Funding details: 74170091 Funding details: 11150807, FONDECYT, Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica Funding details: 1130882, FONDECYT, Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica Funding details: AMS, Agricultural Marketing Service Funding details: OI101205 Funding details: SRC, Semiconductor Research Corporation Funding details: 09MCSS-6691 Funding details: 09MCSS-6698 Funding details: ES Funding text: This study was supported by INNOVA-CORFO (No. 09MCSS-6691 and 09MCSS-6698 ), FONDECYT (No. 1130882 ; 11150807 ), DICYT-USACH , and VRIDEI-USACH (MI, ES, AMS); Universidad Mayor start-Up OI101205 funds to SRC, and the grants from CONICYT-BCH (Chile) Postdoctoral fellowship (No. 74170091 ) to EVV, and VRIDEI-USACH (living/travel allowance) to FERL. 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PY - 2018

Y1 - 2018

N2 - Salmon farming may face stress due to the intensive culture conditions with negative impacts on overall performance. In this aspect, functional feed improves not only the basic nutritional requirements but also the health status and fish growth. However, to date no studies have been carried out to evaluate the effect of functional diets in salmon subjected to crowding stress. Thus, the aim of this study was to evaluate the effect of yeast extract (Xanthophyllomyces dendrorhous; diet A) and the combination of plant extracts (common Saint John's wort, lemon balm, and rosemary; diet B) on the antioxidant and immune status of Atlantic salmon grown under normal cultured conditions and then subjected to crowding stress. Fish were fed with functional diets during 30 days (12 kg/m3) and then subjected to crowding stress (20 kg/m3) for 10 days. The lipid peroxidation in gut showed that both diets induced a marked decrease on oxidative damage when fish were subjected to crowding stress. The protein carbonylation in muscle displayed at day 30 a marked decrease in both functional diets that was more marked on the stress condition. The expression of immune markers (IFNγ, CD4, IL-10, TGF-β, IgMmb, IgMsec, T-Bet, and GATA-3) indicated the upregulation of those associated to humoral-like response (CD4, IL-10, GATA-3) when fish were subjected to crowding stress. These results were confirmed with the expression of secreted IgM. Altogether, these functional diets improved the antioxidant status and increased the expression of genes related to Th2-like response suggesting a protective role on fish subjected to crowding stress. © 2017

AB - Salmon farming may face stress due to the intensive culture conditions with negative impacts on overall performance. In this aspect, functional feed improves not only the basic nutritional requirements but also the health status and fish growth. However, to date no studies have been carried out to evaluate the effect of functional diets in salmon subjected to crowding stress. Thus, the aim of this study was to evaluate the effect of yeast extract (Xanthophyllomyces dendrorhous; diet A) and the combination of plant extracts (common Saint John's wort, lemon balm, and rosemary; diet B) on the antioxidant and immune status of Atlantic salmon grown under normal cultured conditions and then subjected to crowding stress. Fish were fed with functional diets during 30 days (12 kg/m3) and then subjected to crowding stress (20 kg/m3) for 10 days. The lipid peroxidation in gut showed that both diets induced a marked decrease on oxidative damage when fish were subjected to crowding stress. The protein carbonylation in muscle displayed at day 30 a marked decrease in both functional diets that was more marked on the stress condition. The expression of immune markers (IFNγ, CD4, IL-10, TGF-β, IgMmb, IgMsec, T-Bet, and GATA-3) indicated the upregulation of those associated to humoral-like response (CD4, IL-10, GATA-3) when fish were subjected to crowding stress. These results were confirmed with the expression of secreted IgM. Altogether, these functional diets improved the antioxidant status and increased the expression of genes related to Th2-like response suggesting a protective role on fish subjected to crowding stress. © 2017

KW - Atlantic salmon

KW - Crowding stress

KW - Functional feed

KW - Immune response

KW - Oxidative stress

KW - Plant extract

KW - Yeast

U2 - 10.1016/j.fsi.2017.12.061

DO - 10.1016/j.fsi.2017.12.061

M3 - Article

VL - 74

SP - 250

EP - 259

JO - Fish and Shellfish Immunology

T2 - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -